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trypletm express stable trypsin  (Thermo Fisher)


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    Thermo Fisher trypletm express stable trypsin
    Trypletm Express Stable Trypsin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypletm+express+stable+trypsin/Trypsin/us12351650-915-8-13
    Average 99 stars, based on 1 article reviews
    trypletm express stable trypsin - by Bioz Stars, 2026-11
    99/100 stars

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    Cell Culture:

    Article Title: Effect of β-Estradiol on Adipogenesis in a 3T3-L1 Cell Model of Prelamin A Accumulation
    Article Snippet: .. Subsequently, these preadipocytes were tripsinized (TrypLETM Express Stable Trypsin-like Enzyme with Phenol Red; Gibco Life Technologies; Carlsbad, CA, USA) and cultured on 100 mm dishes in DMEM containing 10% FBS and gentamicin 1.05 × 10 −4 mol/L. .. After confluence, 3T3-L1 preadipocytes were induced to differentiate by incubation for 3 days in DMEM differentiation medium containing 10% FBS, insulin (1.74 × 10 −7 mol/L), dexamethasone (2.5 × 10 −7 mol/L), 3-isobutyl-1-methylxanthine (1 × 10 −4 mol/L in DMSO), and a PPARG agonist: pioglitazone (1 × 10 −5 mol/L in DMSO; Enzo Life Sciences, Lausen, Switzerland).

    Article Title: Antibodies binding to fibroblast activation protein alpha and death receptor 4
    Article Snippet: The following day, Hub096 PDOs were dissociated using TrypLETM Express Stable Trypsin-Like Enzyme (Gibco, Cat #12604021) and 1-2 million PDOs were plated on nonadherent 6-Well plates (Corning, Cat #3471) in 6 mL of lentivirus-containing medium (harvested from the virus-producing HEK293T cell culture plate and filtered through a 0.22 μm polyether sulfone filter from Sarstedt B. V., Cat #83.1826.001), supplemented with 6 μg/mL Polybrene® (Sigma-Aldrich, Cat #TR-1003), 0.5 mM N-acetylcysteine (Sigma-Aldrich, Cat #A9165), and 10 μM of ROCK-inhibitor Y-27632 (Abmole bioscience, Cat #HY-10583). .. The following day, Hub096 PDOs were dissociated using TrypLETM Express Stable Trypsin-Like Enzyme (Gibco, Cat #12604021) and 1-2 million PDOs were plated on nonadherent 6-Well plates (Corning, Cat #3471) in 6 mL of lentivirus-containing medium (harvested from the virus-producing HEK293T cell culture plate and filtered through a 0.22 μm polyether sulfone filter from Sarstedt B. V., Cat #83.1826.001), supplemented with 6 μg/mL Polybrene® (Sigma-Aldrich, Cat #TR-1003), 0.5 mM N-acetylcysteine (Sigma-Aldrich, Cat #A9165), and 10 μM of ROCK-inhibitor Y-27632 (Abmole bioscience, Cat #HY-10583). .. After 24 h incubation, PDOs were recovered in 15 mL tubes (Corning, Cat #430791), washed twice with PBS (Corning, Cat #21-031-CV) and resuspended in basement membrane extract (BME) matrix (Amsbio, Cat #3533-010-02) at a 2:1 ratio with PDO medium (refer to Example 11 for medium composition).

    Article Title: Variable Expressivity and Allelic Heterogeneity in Type 2 Familial Partial Lipodystrophy: The p.(Thr528Met) LMNA Variant
    Article Snippet: .. Subsequently, these preadipocytes were trypsinized (TrypLETM Express Stable Trypsin-like Enzyme with Phenol Red; Gibco Life Technologies; Carlsbad, CA, USA) and cultured on 100 mm dishes in DMEM containing 10% FBS and penicillin-streptomycin 1%. .. After confluence, preadipocytes were cultured on 35 mm multi-well dishes (6-well plates) in a differentiation cocktail containing Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum, insulin (1 μg/mL), dexamethasone (0.25 μM) and 3-isobutyl-1-methylxanthine (0.1 mM in DMSO) [ ] for 3 days, with a PPARG agonist, pioglitazone (10 μM in DMSO; Alexis Biochemicals, Lausanne, Switzerland), after which this medium was changed for a growth medium containing 1 μg/mL insulin with pioglitazone (10 μM) for two more days.

    Virus:

    Article Title: Antibodies binding to fibroblast activation protein alpha and death receptor 4
    Article Snippet: The following day, Hub096 PDOs were dissociated using TrypLETM Express Stable Trypsin-Like Enzyme (Gibco, Cat #12604021) and 1-2 million PDOs were plated on nonadherent 6-Well plates (Corning, Cat #3471) in 6 mL of lentivirus-containing medium (harvested from the virus-producing HEK293T cell culture plate and filtered through a 0.22 μm polyether sulfone filter from Sarstedt B. V., Cat #83.1826.001), supplemented with 6 μg/mL Polybrene® (Sigma-Aldrich, Cat #TR-1003), 0.5 mM N-acetylcysteine (Sigma-Aldrich, Cat #A9165), and 10 μM of ROCK-inhibitor Y-27632 (Abmole bioscience, Cat #HY-10583). .. The following day, Hub096 PDOs were dissociated using TrypLETM Express Stable Trypsin-Like Enzyme (Gibco, Cat #12604021) and 1-2 million PDOs were plated on nonadherent 6-Well plates (Corning, Cat #3471) in 6 mL of lentivirus-containing medium (harvested from the virus-producing HEK293T cell culture plate and filtered through a 0.22 μm polyether sulfone filter from Sarstedt B. V., Cat #83.1826.001), supplemented with 6 μg/mL Polybrene® (Sigma-Aldrich, Cat #TR-1003), 0.5 mM N-acetylcysteine (Sigma-Aldrich, Cat #A9165), and 10 μM of ROCK-inhibitor Y-27632 (Abmole bioscience, Cat #HY-10583). .. After 24 h incubation, PDOs were recovered in 15 mL tubes (Corning, Cat #430791), washed twice with PBS (Corning, Cat #21-031-CV) and resuspended in basement membrane extract (BME) matrix (Amsbio, Cat #3533-010-02) at a 2:1 ratio with PDO medium (refer to Example 11 for medium composition).



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